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PowerPoint: Principles of Gel Electrophoresis

SKU: CSPP3 $39.00
PowerPoint Presentation Topics: General Considerations and Principles: The Electrophoretic Matrix: Properties of Agarose and Acrylamide. An Introduction to the theory of Electrophoresis: Interrelationship of Resistance, Voltage, Current and Power, Electrolysis and pH Effects. Nucleic Acid vs. Protein Separation. Denaturing vs. Non-Denaturing (Native) Electrophoresis. Sample Preparation; Comparison of Buffer Systems for Nucleic Acid Separations. Comparison of Buffer Systems for Protein Separation. Detection of Nucleic Acids in Agarose and Acrylamide Gels. Detection of Nucleic Acids on Blot Transfer Membranes. Detection of Proteins in Acrylamide Gels. Detection of Proteins on Blot Transfer Membranes. PowerPoint Presentations: Each 35 - 45 frame presentation contains text in an outline form, charts, graphs and copies of microscopic observations. The material is supported by a manual for the instructor to follow, giving him or her additional information for each frame of the presentation. The presentations are designed to be covered in a 35 - 40 minute class period.
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